Name: Rag1 KO Mouse
Brand: 싸이아젠(Cyagen)
SKU: C001197

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Rag1 KO Mouse

제품명

Rag1 KO Mouse

제품 ID

C001197

품종 계통

C57BL/6NCya-Rag1^em1/Cya

Backgroud

C57BL/6NCya

상태

이 마우스 계통을 논문에서 사용할 경우, “Rag1 KO Mouse (카탈로그 번호 C001197)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.

Other Immunodeficient Mice

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가격 문의

Other Immunodeficient Mice

기본 정보

검증 데이터

The Rag1-KO Mouse is a RAG1-deficient model that exhibits smaller lymphoid organs and does not contain mature B and T lymphocytes, the arrest of B and T cell differentiation occurs at an early stage and correlates with the inability to perform V(D)J recombination. The homozygote Rag1-KO mice developed normally and were fertile.

Reference

Mombaerts P, Iacomini J, Johnson RS, Herrup K, Tonegawa S, Papaioannou VE. RAG-1-deficient mice have no mature B and T lymphocytes. Cell. 1992 Mar 6;68(5):869-77.

Bosticardo M, Pala F, Notarangelo LD. RAG deficiencies: Recent advances in disease pathogenesis and novel therapeutic approaches. Eur J Immunol. 2021 May;51(5):1028-1038.

변형 전략

The Rag1 gene is located on chromosome 2 of the mouse and contains 2 exons, the start codon ATG and the stop codon TAA are both located in Exon 2, and Exon 2 of the Rag1 gene is knocked out by gene editing technology.

Figure 1. Diagram of the gene editing strategy for the generation of Rag1 KO mice.

검증 데이터

1. Detection of T cells and B cell

The peripheral blood (PB), spleen, and thymus of Rag1 KO and wild-type mice were collected, and the composition of T and B cells was analyzed by representative flow cytometric immunophenotype and statistical comparison. An almost complete absence of CD3-CD19+ B cells and CD3+CD19- T cells in peripheral blood (PB), spleen, and thymus of Rag1-KO homozygous mice compared to wild-type mice.

Figure 2. Detection of T and B cells in peripheral blood (PB), spleen, and thymus of Rag1 KO mice.

2. Detection of NK cells, monocytes, macrophages, neutrophils, and dendritic cells (DCs) in peripheral blood

The results show that, compared with WT mice, the proportion of NK cells in Rag1 KO mice increased significantly. There was no significant difference in the proportions of monocytes, macrophages, and dendritic cells (DCs), while the proportion of neutrophils increased.

Figure 3. Flow cytometry results of NK cells, monocytes, macrophages, neutrophils, and dendritic cells (DCs) in the peripheral blood of Rag1 KO mice and wild-type (WT) mice (8-week-old, female, n=3).

3. Detection of NK cells, monocytes, macrophages, neutrophils, and dendritic cells (DCs) in the spleen

The results show that, compared with WT mice, the proportion of NK cells in Rag1 KO mice increased significantly. There was no significant difference in the proportion of monocytes, while the proportions of macrophages, neutrophils, and dendritic cells (DCs) increased.

Figure 4. Flow cytometry results of NK cells, monocytes, macrophages, neutrophils, and dendritic cells (DCs) in the spleens of Rag1 KO mice and wild-type (WT) mice (8-week-old, female, n=3).

4. Detection of NK cells, monocytes, macrophages, neutrophils, and dendritic cells (DCs) in bone marrow

The results show that, compared with WT mice, the proportion of NK cells in Rag1 KO mice increased. There was no significant difference in the proportions of monocytes and macrophages, while the proportions of neutrophils and dendritic cells (DCs) increased slightly.

Figure 5. Flow cytometry results of NK cells, monocytes, macrophages, neutrophils, and dendritic cells (DCs) in the bone marrow of Rag1 KO mice and wild-type (WT) mice (8-week-old, female, n=3).

Rag1 KO Mouse

기본 정보

검증 데이터

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Datasheet

품종 계통 설명

Reference

변형 전략

1. Detection of T cells and B cell

2. Detection of NK cells, monocytes, macrophages, neutrophils, and dendritic cells (DCs) in peripheral blood

3. Detection of NK cells, monocytes, macrophages, neutrophils, and dendritic cells (DCs) in the spleen

4. Detection of NK cells, monocytes, macrophages, neutrophils, and dendritic cells (DCs) in bone marrow

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